Influence of growth rate on the accumulation of ergosterol in yeast-cells |
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Authors: | C. Arnezeder W. A. Hampel |
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Affiliation: | (1) Institute of Biochemical Technology and Microbiology, Technical University of Vienna, Getreidemarkt 9, A-1060 Vienna, Austria |
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Abstract: | Summary The influence of growth rate on the accumulation of ergosterol inSaccharomyces cerevisiae was studied with glucose, maltose, ethanol and acetic acid as substrates under C- and N-limitations in chemostat experiments. In carbon limited cultures an decrease in ergosterol content with rising dilution rate was observed, whereas in nitrogen limited cells an quite opposite behaviour was attained. A maximum specific rate of ergosterol synthesis of about 2 mg per h per g dry cell mass was calculated for nitrogen limited cultures. |
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