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Homoserine and threonine pools of borrelidin resistant Saccharomyces cerevisiae mutants with an altered aspartokinase |
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| Authors: | Michael Seibold Karl Nill Karl Poralla |
| Institution: | (1) Lehrbereich Mikrobiologie I, Institut für Biologie II der Universität, Auf der Morgenstelle 28, D-7400 Tübingen, Germany;(2) Inst. Biologie I, Universität Tübingen, Auf der Morgenstelle 1, D-7400 Tübingen, Germany |
| Abstract: | Borrelidin is a specific inhibitor of the threonyl-tRNA-synthethase. A class of dominant borrelidin resistant mutants (BOR1) of Saccharomyces cerevisiae was biochemically characterized. The mutants possess an altered aspartokinase which is insensitive to threonine inhibition. The threonine and homoserine pools in these mutants are 22 times larger than in the wild type. By feeding aspartate under a variety of conditions the homoserine pool is increased even 57 times whilst the threonine pool is reduced. |
| Keywords: | Saccharomyces cerevisiae Borrelidin Aspartokinase Feedback regulation Threonine pool Homoserine pool S-Adenosylmethionine |
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