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Paraquat Resistant Tobacco Calluses with Enhanced Superoxide Dismutase Activity |
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| Authors: | Furusawa Iwao; Tanaka Kunisuke; Thanutong Porntip; Mizuguchi Atsuo; Yazaki Motoki; Asada Kozi |
| Institution: | 1Laboratory of Plant Pathology, Faculty of Agriculture, Kyoto University Kyoto 606, Japan 2Department of Biochemistry, College of Agriculture, Kyoto Prefectural University Kyoto 606, Japan 3The Research Institute for Food Science, Kyoto University Uji, Kyoto 611, Japan |
| Abstract: | Seven paraquat resistant calluses of tobacco (Nicotiana tabacumL. cv. Samsun) were obtained by three successive screeningsof protoplast-derived calluses on a paraquat containing medium.Superoxide dismutase (SOD) activity of the resistant calluseswas 14- to 159-fold that of the leaf cells on protein basis.Paraquat-resistant calluses, however, showed little increasein catalase and peroxidase activities. More than 90% of SODactivity in the resistant calluses was inhibited by KCN, aswas the SOD activity in leaves, indicating that the major SODin the callus appears to be the Cu, Zn containing enzyme. Thecallus cells, however, expressed the immunologically distinguishedSOD isozyme from the enzyme in the leaves. (Received April 23, 1984; Accepted August 6, 1984) |
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