Overexpression of a NTR1 in transgenic soybean confers tolerance to water stress |
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Authors: | Ren-Gao Xue Biao Zhang Hong-Feng Xie |
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Institution: | (1) Department of Life & Science, Laiyang Agricultural College, Qingdao, 266109, China |
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Abstract: | Methyl jasmonate (MeJA) is an important plant regulator that involves in plant development and regulates the expression of
plant defense genes in response to various stresses such as wounding, drought, and pathogens. In order to determine the physiological
role of endogenous MeJA in plants, a NTR1 from Brassica campestris encoding a jasmonic acid carboxyl methyltransferase that produces methyl jasmonate was constructed under the control of CaMV
35S promoter and transformed into soybean Glycine max (L) Merrill]. The transgenic soybean plants constitutively expressed the NTR1 and accumulated more MeJA levels than wild type plants. Overexpression of the gene in transgenic soybean conferred tolerance
to dehydration during seed germination and seedling growth as reflected by the percentage of the fresh weight of seedlings.
In addition, the transgenic soybean plants also conferred better capacity to retain water than wild type plants when drought
tolerance was tested using detached leaves. |
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Keywords: | Drought tolerance Methyl jasmonate NTR1 Soybean Water stress |
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