抗—HBs国际单位RIA一点法定量测定与计算方法的探讨

在Radio-immuno Assay(RIA)试验测抗－HBs国际单位的简易定量与计算中，采用RIA一点法测定，以分析表达式mIU/ml＝SC（mIU/ml)[exp(0.69315S-NC/SC-NC-1]进行结果计算。此式可以常用对数式表示为mIU/ml=SC(mIU/ml)[lg^-1(0.30103S-NC/SC-NC-1],当（S－NC）在0．3－1．1界线内时，其计算结果的相对误差小于6％，是目前误差最小的简易计算方法。同时还推算求出Holliger公式的阳性对照的最适含量为124．26mIU／ml,Richardson公式的阳性对照为125mIU/ml，中国药品生物制品检定所公式的阳性对照为92．5mIU/ml。

Study of quantitative assay and calculate method with RIA A dot-assay in titrating IU of anti-HBs

In the study of simple quantitative assay and calculate method with Radio Immuno Assay(RIA) in titrating IU of anti HBs,the RIA A Dot Assay method was carried out and calculated the result with the formula of:mIU/ml=SC(mIU/ml)[exp(0.69315S-NCSC-NC)-1]Which could be expressed by log formula as:mIU/ml=SC(mIU/ml)[log( 0.30103 S-NCSC-NC)-1] When(S-NC)/(SC-NC)within the range of 0.3-1.1,the relative error was <6%.Up to now,it is a simple method with the least error as well known.In this article,author calculated that:124.26 mIU/ml were as the most proper content of positive control of Holliger formula;125 mIU/ml,of Richardson formula;and 92.5mIU/ml,of the formula from NICPBP(National Institute for the Control of Pharmaceutical and Biological Products) respectively.