Antioxidant properties of alpha-crystallin |
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Authors: | Manzanares D Bauby C de la Peña R Garcia J C Sanchez R Martinez S Romay C H López-Reconde J L Pino E Lissi E A |
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Institution: | (1) Centro de Investigaciones Biomédicas (CIBIOMED), Instituto Superior de Ciencias Médicas de la Habana, Havana, Cuba;(2) Centro Nacional de Investigaciones Cientificas, CNIC, Havana, Cuba;(3) Centro de Ingeniería Genética y Biotecnologia, Havana, Cuba;(4) Facultad de Química y Biologia, Universidad de Santiago, Santiago, Chile |
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Abstract: | -Crystallin is a major chaperone lens protein to which has been ascribed antioxidant functions. In the present work we have evaluated the antioxidant and free radical scavenging properties of bovine -crystallin in a series of in vitro models: zimosan-induced, luminol-enhanced chemiluminescence response of polymorphonuclear leukocytes, the autoxidation of brain homogenate, bleaching of 2,2 -azinobis(3-ethylbenzothiazoline-6-sulfonic acid)-derived radical cations, trapping of peroxyl radicals, and reactivity toward hypochloric acid. In all these systems, the reactivity of -crystallin is higher than or similar to that of bovine serum albumin. It is concluded that, given the high concentrations of -crystallin in the lenses, its capacity to interact with free radicals and to remove hypochlorous acid could contribute to the maintenance of the lens functionality. |
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Keywords: | -Crystallin" target="_blank">gif" alt="agr" align="BASELINE" BORDER="0">-Crystallin antioxidant activity peroxyl radicals hypochlorite brain homogenate autoxidation |
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