| 黑曲霉糖化酶基因启动子功能鉴定 |
| |
| 引用本文： | 李昆,李松,牛丹丹,王正祥.黑曲霉糖化酶基因启动子功能鉴定[J].微生物学杂志,2008,28(6):5-9. |
| |
| 作者姓名： | 李昆 李松 牛丹丹 王正祥 |
| |
| 作者单位： | 江南大学生物工程学院工业,生物技术教育部重点实验室,江苏,无锡,214122 |
| |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| |
| 摘 要： | 从糖化酶工业生产菌株Aspergillus nigerCICIM F0410基因组DNA中扩增糖化酶基因启动子(PglaA),并将该启动子替换质粒pRS303K上KmR基因启动子,构建成糖化酶基因启动子功能检测质粒pRS-PglaA-KmR。将pRS-PglaA-KmR转入E.coliJM109中,得到重组菌E.coli(pRS-PglaA-KmR)。通过对重组菌的氨基糖苷磷酸转移酶基因活性检测,表明PglaA在E.coli中具有驱动KmR基因表达的活性。采用不同诱导物进行培养发现,葡萄糖、蔗糖、乳糖、麦芽糖或玉米淀粉,可以不同程度增强PglaA的强度。
|
| 关 键 词： | 黑曲霉 糖化酶基因启动子 功能鉴定 |
Function Characterization of Glucoamylase Gene Promoter from Aspergillus niger CICIM F0410 |
| |
| LI Kun,LI Song,NIU Dan-dan,WANG Zheng-xiang.Function Characterization of Glucoamylase Gene Promoter from Aspergillus niger CICIM F0410[J].Journal of Microbiology,2008,28(6):5-9. |
| |
| Authors: | LI Kun LI Song NIU Dan-dan WANG Zheng-xiang |
| |
| Abstract: | Glucoamylase gene promoter(PglaA) from genome DNA of industrial Aspergillus nigerstrain CICIM F0410 for producing the enzyme was amplified and replace KmRpromoter in plasmid pRS303K with it to construct a glucoamylase gene promoter function testing plasmid pRS-PglaA-KmR.And the testing plasmid was transferred into E.coliJM109 and obtained recombinant E.coli(pSR-PglaA-KmR).Through the examination the activity of amino-glucoside phosphate transferring enzyme of the recombinant bacterium indicated that PglaA possessed the activity to drive the genetic expression of KmR.It was found that culture adopting with different inducers,glucose,sucrose,lactose,maltose,and corn starch,the strength of PglaA was enhanced to different degrees. |
| |
| Keywords: | Aspergillus niger promoter of glucoamylase gene function characterization |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《微生物学杂志》浏览原始摘要信息 |
| 点击此处可从《微生物学杂志》下载免费的PDF全文 |
|
