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Estimation of pullulan by hydrolysis with pullulanase |
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| Authors: | Sheng-Jun Wu Jin-Moon Kim Chao Zhou Zheng-Yu Jin Qun-Yi Tong |
| Institution: | 1. School of Food Engineering, HuaiHai Institute of Technology, Lianyungang, China 2. Food Safety and Inspection Service, USDA, 230 Washington Avenue Extension, Albany, NY, 12203, USA 3. The State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, China |
| Abstract: | A novel method for the estimation of pullulan was developed in which pullulan was hydrolysed by pullulanase. The hydrolysed product was mainly maltotriose and was determined colorimetrically using 3,5-dimethylsalicylic acid. This gave good linearity with respect to the concentration of pullulan in the fermentation broth. The content of pullulan determined in this way was less than that determined by a coupled enzyme assay and was comparable to that determined by an HPLC method. The new method was specific for estimation of pullulan, demonstrated high accuracy, and could assay pullulan from up to 3.2 mg/ml. |
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