Divergent selection for in vitro developmental capacity of preimplantation mouse embryos |
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Authors: | D. Pomp E. S. Critser J. J. Rutledge |
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Affiliation: | (1) Department of Meat and Animal Science, University of Wisconsin, 53706 Madison, WI, USA;(2) Present address: Department of Animal Science, North Carolina State University, Box 7621, 27695-7621 Raleigh, NC, USA;(3) Present address: Methodist Center for Reproduction and Transplantation Immunology, Methodist Hospital, 46202 Indianapolis, IN, USA |
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Abstract: | Summary Replicated divergent selection was conducted for two generations in ICR mice for in vitro developmental capacity (IVDC; percentage of fertilized one-cell zygotes developing to blastocysts in vitro per female donor). Realized heritabilities based on high and low selection were 0.03±0.08 and –0.11±0.09 in replicate 1, and 0.10±0.11 and 0.08±0.10 in replicate 2. No differences were detected between selection lines (P>0.2) or replicates (P>0.1). Estimate of heritability in the base population based on 332 daughter-dam pairs was 0.14±0.18. These results indicate that additive genetic variance contributes little to the phenotypic variance in this trait. Considerable phenotypic variation in IVDC was observed (mean=49.3; SD=31.0), with a range of IVDC from 0%–100%. Utilization of donor female as a blocking factor is suggested for designs of experiments with preimplantation embryos to increase precision and power of statistical analyses. |
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Keywords: | Selection Embryo development Embryo culture Mice |
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