将云南原小单孢菌转入纤维化纤维菌的多相分类研究

对中国普通微生物菌种保藏中心(CGMCC)保藏的云南原小单孢菌(Promicromonospora yunnanensis)AS4.1333进行的多相分类研究表明,菌株AS4.1333与纤维化纤维菌(Cellulosimicrobium cellulans)DSM43879T关系密切,它们的16S rRNA基因序列相似性为99.6%,DNA同源性为89.3%。胞壁组分、枝菌酸、甲基萘醌、磷酸类脂和DNAG Cmol%测定等化学分类结果支持了上述结论。在形态和生理生化特性上,菌株AS4.1333与CellulosimicrobiumcellulansDSM43879T之间也表现出非常相似的性状。根据系统发育分析、化学分类、形态和生理生化特性、DNA同源性测定等研究结果,对菌株AS4.1333的分类地位做了修正,将其从原小单孢菌属中排除,认为菌株AS4.1333与Cellulosimicrobium cellulansDSM43879T是同一个种,建议将菌株AS4.1333由云南原小单孢菌(Promicromonosporayunnanensis)转入纤维化纤维菌(Cellulosimicrobium cellulans)。

Polyphasic evidence for the transfer of Promicromonospora yunnanensis to Cellulosimicrobium cellulans

Polyphasic taxonomic investigations of Promicromonospora yunnanensis AS4.1333 deposited in the China General Microbiological Culture Collection Center(CGMCC) indicated that strain AS4.1333 was closely related to Cellulosimicrobium cellulans DSM43879~T,the two organisms shared a 16S rRNA gene similarity of 99.6% which correspond to 5 nt differences at 1423 positions.Corresponding DNA-DNA reassociation value was 89.3%,significantly higher than 70% cut-off point recommended for the delineation of genomic species by Wayne et al.(1987).Results of chemotaxonomic analyses of cell wall(Whole-organism hydrolysates were rich in rhamnose,fucose and galactose;peptidoglycan type A4a),mycolic acids(One dimensional TLC of whole-organism acid methanolysates revealed the absence of a lower spot(Rf value around 0.47) that corresponded to mycolic acids),principal menaquinones(MK-9(H-4)),phospholipid type(PV)and the G C content of the DNA(73.8 mol%) supported the conclusions of the genotypic analyses.The very similar morphological and physiological characteristics agreed with the high degree of relatedness.On the basis of phylogenetic analyses based on the almost complete 16S rRNA gene sequence,DNA-DNA reassociation values,chemotaxonomic properties,morphological and physiological characteristics,it is concluded that strain AS4.1333 should be removed from the genus Promicromonospora,and strain AS4.1333 and Cellulosimicrobium cellulans should be considered to be a single species.Promicromonospora yunnanensis AS4.1333 was proposed to transfer into Cellulosimicrobium cellulans.The type strain remains DSM43879~T.