19F labelled glycosaminoglycan probes for solution NMR and non-linear (CARS) microscopy |
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| Authors: | Marcelo A. Lima Renan P. Cavalheiro Gustavo M.Viana Maria C.Z. Meneghetti Timothy R. Rudd Mark A. Skidmore Andrew K. Powell Edwin A. Yates |
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| Affiliation: | 1.Department of Biochemistry,UNIFESP,S?o Paulo,Brazil;2.Department of Biochemistry,University of Liverpool,Liverpool,UK;3.The National Institute of Biological Standards and Controls, Blanche Lane,Potters Bar,UK;4.School of Life Sciences,Keele University,Keele,UK;5.School of Pharmacy and Biomolecular Sciences,Liverpool John Moores University,Liverpool,UK |
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| Abstract: | Studying polysaccharide-protein interactions under physiological conditions by conventional techniques is challenging. Ideally, macromolecules could be followed by both in vitro spectroscopy experiments as well as in tissues using microscopy, to enable a proper comparison of results over these different scales but, often, this is not feasible. The cell surface and extracellular matrix polysaccharides, glycosaminoglycans (GAGs) lack groups that can be detected selectively in the biological milieu. The introduction of 19F labels into GAG polysaccharides is explored and the interaction of a labelled GAG with the heparin-binding protein, antithrombin, employing 19F NMR spectroscopy is followed. Furthermore, the ability of 19F labelled GAGs to be imaged using CARS microscopy is demonstrated. 19F labelled GAGs enable both 19F NMR protein-GAG binding studies in solution at the molecular level and non-linear microscopy at a microscopic scale to be conducted on the same material, essentially free of background signals. |
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