Degradation and replenishment of the labile protein fraction in non-growing cells of the asporogenic mutant ofBacillus megaterium |
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Authors: | J Chaloupka M Strnadová |
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Institution: | (1) Department of Cell Physiology, Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20 Prague 4 |
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Abstract: | Kinetics of degradation of labelled proteins was followed in two asporogenic mutants ofBacillus megaterium during incubation in a sporulation medium. Both the mutant producing exocellular protease (KM 1prn
+) and the mutant not producing the enzyme (KM 12prn) were found to contain a labile protein fraction, whose proportion decreases with prolonged time of labelling and whose half-life
is about 1 h. Most proteins were relatively stable and were degraded at a rate of 1 %/h and 2 %/h in strains KM 1 and KM 12,
respectively (half life 70–80 h and 35–40 h in strains KM 1 and KM 12, respectively). The intracellular proteolytic activity
of the KM 12 mutant remains practically the same during incubation in the sporulation medium or slowly increases. The labile
protein fraction practically disappears from the cells after a 3.5-h incubation. When such a culture is then subjected to
a shift-up and transferred again to the sporulation medium, the rate of protein turnover temporarily increases. The temporary
increase of the turnover rate is caused by a partial replenishment of the labile protein fraction rather than by an accelerated
degradation of the relatively stable fraction. The intracellular proteolytic activity does not increase under these conditions.
The wild sporogenic strain ofB. megaterium also contains the labile protein fraction. Its half protein life is 1 h or less. However, the second protein fraction is
degraded much more rapidly than in the asporogenic mutants and its half life is 6–7 h. |
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