SSR based linkage and mapping analysis of C,a yellow cocoon gene in the silkworm,Bombyx mori |
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| Authors: | Yun‐Po Zhao Mu‐Wang Li An‐Ying Xu Cheng‐Xiang Hou Ming‐Hui Li Qiu‐Hong Guo Yong‐Ping Huang Xi‐Jie Guo |
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| Affiliation: | 1. Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, Jiangsu Province, China;2. College of Biotechnology and Environmental Engineering, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu Province, China;3. Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China |
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| Abstract: | The yellow color of the cocoon of the silkworm Bombyx mori is controlled by three genes, Y (Yellow haemolymph), I (Yellow inhibitor) and C (Outer‐layer yellow cocoon), which are located on linkage groups 2, 9 and 12, respectively. Taking advantage of a lack of crossing over in females, reciprocal backcrossed F1 (BC1) progeny were used for linkage analysis and mapping of the C gene using silkworm strains C108 and KY, which spin white and yellow cocoons, respectively. DNA was extracted from individual pupae and analyzed for simple sequence repeat (SSR) markers. The C gene was found to be linked to seven SSR markers. All the yellow cocoon individuals from a female heterozygous backcross (BC1 F) showed a heterozygous profile for SSR markers on linkage group 12, whereas individuals with light yellow cocoons showed the homozygous profile of the strain C108. Using a reciprocal heterozygous male backcross (BC1 M), we constructed a linkage map of 36.4 cM with the C gene located at the distal end, and the closest SSR marker at a distance of 13.9 cM. |
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| Keywords: | Bombyx mori carotenoid uptake C gene linkage map SSR markers |
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