Effects of free and chelated iron on in vitro androgenesis in barley and wheat |
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Authors: | Novotný Jiří Vagera Jiří Ohnoutková Ludmila |
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Affiliation: | (1) Faculty of Science, Department of Cell Biology and Genetics, Palacky University, Šlechtitelů 11, CZ-783 71 Olomouc, Czech Republic;(2) Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Sokolovská 6, CZ-772 00 Olomouc, Czech Republic |
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Abstract: | A suitable form of iron supplement in the induction medium was found to be important for further development of induced pollen embryos in barley and wheat cultivars (genotypes), especially those providing few green plants viain vitro androgenesis. Genotypes able to regenerate many green plants were less susceptible to the lack of iron in induction medium. Although Fe-EDTA was found to be a suitable form of iron in the induction medium, androgenesis was also induced on media containing non-chelated iron (Fe2+ and Fe3+ ions). EDTA alone without iron inhibited the androgenic response even in the wheat cv. Florida, a model cultivar for androgenesis in wheat. In all barley cultivars under study including cv. Igri, a model cultivar for androgenesis in barley, EDTA alone caused an almost total suppression of androgenesis. This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | anther culture EDTA ferrous ions ferric ions Hordeum vulgare Triticum aestivum |
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