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Light Dependent Reduction of Pertechnetate (TcO(4)) by Broken Chloroplasts
Authors:Lembrechts J F  Desmet G M
Institution:Rijksinstituut voot Volksgezondheid en Milieuhygiëne, c/o Stichting ITAL Keyenbergseweg 6, 6704 PJ Wageningen, The Netherlands;Laboratory of Radiation Research, c/o Stichting ITAL Keyenbergseweg 6, 6704 PJ Wageningen, The Netherlands
Abstract:Arguments are given for a ferredoxin-mediated reduction of TcO4, preponderantly into extractable Tc(V) complexes, by illuminated, broken chloroplasts. Photosynthetic O2- and NADP-reduction competitively inhibit Tc incorporation. As for O2, the reaction can be stimulated by the auto-oxidizable electron acceptor methyl viologen. Furthermore TcO4 can function as terminal acceptor in the diaphorase reaction, with NADPH as electron donor.
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