Mycoparasitism studies of Trichoderma harzianum strains against Rhizoctonia solani: evaluation of coiling and hydrolytic enzyme production |
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Authors: | Fausto Bruno dos Reis Almeida Fernanda Menezes Cerqueira Roberto do Nascimento Silva Cirano José Ulhoa Adilson Lopes Lima |
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Affiliation: | (1) Laboratório de Enzimologia, Universidade Federal de Goiás (ICB), 74001-970 Goiania, GO, Brazil;(2) Embrapa Amapá, Rodovia Juscelino Kubitschek, Km 5, Caixa Postal 10, 68903-000 Macapá, AP, Brazil |
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Abstract: | The genus Trichoderma is a potential biocontrol agent against several phytopathogenic fungi. One parameter for its successful use is an efficient coiling process followed by a substantial production of hydrolytic enzymes. The interaction between fifteen isolates of Trichoderma harzianum and the soil-borne plant pathogen, Rhizoctonia solani, was studied by light microscopy and transmission electron microscopy (TEM). Macroscopic observations of fungal growth in dual cultures revealed that growth inhibition of the pathogen occurred soon after contact with the antagonist. All T. harzianum isolates tested exhibited coiling around the hyphae of R. solani. The strains ALL23, ALL40, ALL41, ALL43 and ALL49 did not differ in coiling frequency and gave equal coiling performances. No correlation between coiling frequency and the production of cell wall-degrading chitinases, N-acetyl-β-d-glucosaminidase and β-1,3-glucanases, was found. |
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Keywords: | N-acetyl-β - font-variant:small-caps" >d-glucosaminidase Chitinases β -1,3-glucanases Mycoparasitism Trichoderma harzianum |
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