Karyotype analysis of Nicotiana kawakamii Y. Ohashi using DAPI banding and rDNA FISH |
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| Authors: | R Nakamura S Kitamura M Inoue N Ohmido K Fukui |
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| Institution: | (1) Laboratory of Plant Breeding Science, Faculty of Agriculture, Kyoto Prefectural University, Sakyo, Kyoto 606-8522, Japan Fax: +81-75-7035603, JP;(2) Laboratory of Rice Genetic Engineering, Hokuriku National Agricultural Experiment Station, 1-2-1 Inada, Joetsu 943-0193, Japan, Present address: K. Fukui, Department of Biotechnology, Faculty of Engineering, Graduate School of Osaka University, 2-1 Yamada-Oka, Suita 565-0871, Japan, JP |
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| Abstract: | Prometaphase cells were used to analyze the karyotype of Nicotiana kawakamii Y. Ohashi by means of sequential Giemsa/CMA/DAPI staining and multicolor fluorescence in situ hybridization with 5S and 18S
rDNA. Observation of the DAPI-stained prometaphase spreads indicated that N. kawakamii had six pairs of large chromosomes, one pair of medium-sized chromosomes and five pairs of small chromosomes. The six pairs
of large chromosomes possessed remarkable DAPI bands, and each could be identified from both the DAPI banding pattern and
the length of the short arm. The DAPI banding pattern was approximately identical to the CMA and Giemsa banding patterns.
Hybridization signals of the 18S rDNA probe were detected on two pairs of large chromosomes. In addition, two pairs of small
chromosomes were identified based on the position of the 5S rDNA signals. An idiogram of N. kawakamii chromosomes was produced based on DAPI bands and rDNA loci.
Received: 17 July 2000 / Accepted: 4 September 2000 |
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| Keywords: | Nicotiana kawakamii Y Ohashi Karyotype Giemsa/CMA/DAPI staining rDNA FISH Quantitative idiogram |
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