Structure of the histone tetramer (H3-H4)2: 1. Peculiarities of optical absorption,fluorescence and light scattering in response to changes in ionic strength and pH of the medium |
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| Authors: | S.N. Khrapunov A.I. Dragan A.F. Protas G.D. Berdyshev |
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| Affiliation: | Department of General and Molecular Genetics, Kiev State Universty, Kiev, USSR |
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| Abstract: | Using the methods of spectrophotometry, spectrofluorimetry, light scattering and gel filtration, it was shown that, at pH 5.6 and 7.4 and various ionic strengths, the histone tetramer (H3-H4)2 may have several structural states with different packing of the polypeptide chains of histones H3 and H4. Two structural changes of the tetramer (H3-H4)2 at pH 7.4 in the ranges 0.1–0.3 m and 0.7–0.9 m NaCl were observed. In the high ionic strength solution, the tetramer (H3-H4)2 had a more compact structure at pH 7.4 than at pH 5.6. At pH 3.0 destruction of the histone tetramer (H3-H4)2 and formation of non-specific aggregates took place. |
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| Keywords: | Fluorescence histone oligomers absorbance quantum yield protein fluorescence anisotropy histone conformers hypochromic effect |
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