X-ray scattering of antibodies: the monomeric 8S subunit of human IgM |
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| Authors: | Peter Wilhelm Ingrid Pilz Erika Schwarz Constantin Mihaesco Edith Mihaesco |
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| Institution: | Institut für Physikalische Chemie der Universität Graz, A-8010 Graz, Heinrichstrasse 28, Austria;Laboratoire d''Immunochimie et d''Immunopathologie, INSERM U. 108, Hópital Saint-Louis, 2 place du Docteur Fournier, F-75475 Paris, France |
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| Abstract: | 8S monomeric subunit of a human immunoglobulin M was investigated by small-angle X-ray scattering. The following molecular parameters were determined: radius of gyration 5.9 nm, maximum length 21 nm, hydrated volume 410 nm3 and two radii of gyration of the cross-section: 2.6 and 1.8 nm. A model equivalent in scattering was found and compared with the model for a human IgG also based on small-angle X-ray scattering data. The Cμ2 domain of the IgM obviously has a very loose structure, and the Fab angle of the 8S IgM (90°) is smaller than that of the IgG (134°). |
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| Keywords: | Antibodies IgM subunit small-angle scattering |
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