Effects of molecular weight of dextran and NAD+ density on coenzyme activity of high molecular weight NAD+ derivative covalently bound to dextran |
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Authors: | S. Adachi M. Ogata H. Tobita K. Hashimoto |
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Affiliation: | Department of Chemical Engineering, Kyoto University, Kyoto 606, Japan |
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Abstract: | NAD+ has been covalently attached to dextrans having different molecular weights to give various NAD+ densities (mol NAD+ per mol d-glucosyl residue). The effects of molecular weight of dextran and of NAD+ density on the coenzyme activity of the dextran-bound NAD+ derivatives were examined for the reactions catalysed by alcohol dehydrogenase (alcohol: NAD+ oxidoreductase, EC 1.1.1.1) and lactate dehydrogenase (l-lactate:NAD+ oxidoreductase, EC 1.1.1.27). The molecular weight of dextran had little effect on coenzyme activity in the range 10 000 to 500 000. At low NAD+ density (<0.05 mol NAD+/mol d-glucosyl residue), the coenzyme activities of the derivatives were relatively low, but higher densities had little effect on the activity. Dextran-bound NAD+ derivatives were twice as stable as free NAD+. |
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Keywords: | Enzymes coenzymes alcohol dehydrogenase EC 1.1.1.1 lactate dehydrogenase EC 1.1.1.27 dextran |
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