Isolating conifer DNA: A superior polysaccharide elimination method |
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Authors: | Crowley Tamsyn M. Muralitharan Morley S. Stevenson Trevor W. |
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Affiliation: | (1) School of Biological and Chemical Sciences, Deakin University, 3217 Geelong, Victoria, Australia |
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Abstract: | Genomic DNA was isolated from frozen needles of maturePinus radiata clones using a modified extraction technique incorporating cetyltrimethylammonium bromide (CTAB) for cell lysis. A high sodium chloride concentration (2 M) was used at 2 stages of the extraction procedure to eradicate polysaccharides, yielding pure genomic DNA suitable for restriction enzyme digestion and PCR amplification. Extractions were scaled down to suit 1.5-mL Eppendorf tubes, allowing easier handling and enhanced sterility. |
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Keywords: | conifer CTAB genomic DNA Pinus radiata polysaccharides |
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