Study of the population dynamics of human diploid cell strain WI-38. III. Serial cultivation of the cells |
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| Authors: | D Slonim |
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| Affiliation: | 1. Connaught Medical Research Laboratories, Toronto, Canada;1. Division of Cardiology, Department of Pediatrics, University of Washington School of Medicine and Seattle Children’s Hospital, Seattle, Washington;2. Department of Research Informatics, University of Washington School of Medicine and Seattle Children’s Hospital, Seattle, Washington;1. Department of Dermatology, Medical College of Wisconsin, Milwaukee, WI, USA;2. Autonomous University of Zacatecas, Zacatecas, Mexico;3. Faculty of Odontology, School of Biomedical Sciences of the Cuauhtémoc University Aguascalientes, Aguascalientes, Mexico;4. School of Life Sciences, University of Warwick, Coventry, UK;1. Department of Chemistry, Faculty of Technology and Sciences, Lovely Professional University, Phagwara, Punjab, India;2. Department of Chemistry, Islamic University of Science and Technology, Awantipora, J&K, India;3. Universidad Andres Bello, Facultad de Ciencias Exactas, Departamento de Ciencias Químicas, Viña del Mar, Chile;4. Center of Applied Nanoscience (CANS), Facultad de Ciencias Exactas, Universidad Andres Bello, Santiago, Chile;5. Relativistic Molecular Physics Group (ReMoPh), PhD Program in Molecular Physical Chemistry, Facultad de Ciencias Exactas, Universidad Andres Bello, Santiago, Chile;6. Universidad de Santiago de Chile (USACH), Facultad de Química y Biología, Departamento de Ciencias del Ambiente, Santiago, Chile;7. Laboratorio de Bioingeniería Molecular a Multiescala, Facultad de Ciencias, Universidad Autónoma del Estado de México, Av. Instituto Literario 100, 50000 Toluca, México;8. Facultad de Ingeniería y Negocios, Universidad de las Américas, Santiago 7500000, Chile;9. Center of New Drugs for Hypertension (CENDHY), Santiago 8380494, Chile;10. Department of Chemistry, Himachal Pradesh University, Summer Hill, Shimla, Himachal Pradesh 171005, India;11. NCE, Department of Science and Technology, Government of Bihar, India |
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| Abstract: | When a 1 : 2 passage system was used the lifespan of WI-38 cells was the same in Basal Medium Eagle (BME) containing glucose and in BME containing galactose and sodium pyruvate; in both cases 33 population doublings were reached before the final ‘Phase III’ occurred. In both cases also the maximum cell yields decreased continuously during serial cultivation. In BME containing glucose the decrease was 0·011 log10 per generation and using BME containing galactose and sodium pyruvate substituted for glucose it was 0·015 log10.On the other hand, if a constant and reduced inoculum of 104·2 cells/cm2 was used at each transfer the lifespan of WI-38 cells was about the same (i.e. 34 population doublings) in BME containing galactose and sodium pyruvate substituted for glucose, but in BME containing glucose it was longer by about seven population doublings. The maximum cell yields in the medium with galactose and sodium pyruvate present decreased by 0·062 log10 and in the medium containing glucose by 0·015 log10 per population generation. |
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