Asb4, Ata3, and Dcn are novel imprinted genes identified by high-throughput screening using RIKEN cDNA microarray |
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Authors: | Mizuno Yosuke Sotomaru Yusuke Katsuzawa Yukiko Kono Tomohiro Meguro Makiko Oshimura Mitsuo Kawai Jun Tomaru Yasuhiro Kiyosawa Hidenori Nikaido Itoshi Amanuma Hiroshi Hayashizaki Yoshihide Okazaki Yasushi |
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Affiliation: | Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center, RIKEN Yokohama Institute, Yokohama 230-0045, Japan. |
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Abstract: | Genes differentially expressed between parthenogenetic and androgenetic embryos are candidates for the identification of imprinted genes, which are expressed specifically from the maternal or paternal allele. To search for genes differentially expressed between parthenogenetic and androgenetic embryos, we used the RIKEN full-length enriched mouse cDNA microarray. The 25 candidates obtained included 8 known imprinted genes (such as IgfII, Snrpn, and Neuronatin) and 3 new ones--Asb4 (ankyrin repeat and SOCS box-containing protein 4), Ata3 (amino acid transport system A3), and Decorin--which were confirmed by using normal diploid embryos from the reciprocal F1 crosses of B6 and JF1 mice. The 25 candidates also included genes that showed no imprinting-associated expression in normal diploid embryos. We describe a feasible high-throughput method of screening for novel imprinted genes by using the RIKEN cDNA microarray. |
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Keywords: | cDNA microarray imprinted gene androgenetic embryo parthenogenetic embryo Asb4 Ata3 Dcn. |
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