Direct evidence for the non-random localization of mammalian chromosomes in the interphase nucleus |
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| Authors: | G Hadlaczky M Went N R Ringertz |
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| Affiliation: | 1. Department of Medical Cell Genetics, Medical Nobel Institute S-10401 Stockholm, Sweden;2. Department of Dermatology, Karolinska Institutet, S-10401 Stockholm, Sweden;1. Department of Biological Sciences, Rutgers University, Newark, NJ, 07102, United States;2. Department of Earth and Environmental Science, Rutgers University, Newark, NJ, United States;3. National Ecological Observatory Network, Battelle, 1685 38th Street Suite 100, Boulder, CO, 80301, United States;1. Third Institute of Oceanography, Ministry of Natural Resources, China;2. Research Centre for Oceanography, Indonesian Institute of Sciences, Indonesia;3. Gili Matra Marine Park Management Office, Indonesia;1. University of Florida, Department of Biochemistry and Molecular Biology, Center for Structural Biology, The McKnight Brain Institute, Gainesville, FL 32610, USA;2. Gene Therapy Program, Department of Pathology and Laboratory Medicine, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA 19104, USA |
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| Abstract: | Indirect immunofluorescence staining with human anti-centromere autoantibodies from a patient (LU 851) suffering from the CREST form of scleroderma was used to analyse chromosome topology in interphase nuclei of rat-kangaroo (PTO) and Indian muntjac (IM) cells. In some cells, centromeres were arranged in pairs suggesting association of homologous chromosomes. Clustering of centromeres at one pole of the nucleus (Rabl configuration) and other patterns suggesting higher order organization were also observed. In one fifth of the IM cells it was possible to identify the intranuclear location of each single chromosome on the basis of the morphology of the immunostained centromeres. In 30% of the IM cells in which centromeres could be identified, homologous chromosomes occupied adjacent territories within the interphase chromatin. |
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