A β-lactamase produced by a thermophilic Bacillus |
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| Authors: | Fouzia Rhazi-Filali,Abdelhamid Zaid,Philippe Ledent,Marc Vanhove,Jozef van,Beeumen Jean-Marie Frè re |
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| Affiliation: | Facultédes Sciences, Universitéde Meknes, P.O. Box 4010, Meknes, Morocco; Centre for Protein Engineering, Universitéde Liège, Institut de Chimie, B6, Sart-Tilman, B4000 Liege, Belgium; Vakgroep Biochemie, Fysiologie en Microbiologie, Unirersiteit Gent, Ledeganckstraat 35, B9000 Ghent, Belgium |
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| Abstract: | Abstract A β-lactamase was purified from a thermophilic Bacillus strain, that had been isolated from a traditional hot bath in the Meknes area (Morocco). The properties of the enzyme were very similar to those of the β-lactamase produced by Bacillus licheniformis 749C but it exhibited a somewhat increased thermostability and a higher activation energy with cefazolin as substrate. These properties were expected for an enzyme produced by a thermophilic strain. |
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| Keywords: | Thermophile Bacillus β-Lactamase Penicillin Cephalosporin |
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