Expression and location of caffeine synthase in tea plants |
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Authors: | Yuan-Hua Li Wei Gu Sheng Ye |
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Institution: | (1) College of Life Sciences, Wuhan University, Wuhan, 430072, China;(2) College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China;(3) Department of Biology and Chemistry, City University of Hong Kong, Kowloon, Hong Kong, China |
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Abstract: | Tea caffeine synthase (CS) is a key enzyme in tea plant for synthesis of caffeine. We firstly systematically investigated
the gene expression of CS. Northern blot analysis showed that CS gene was not expressed in stems and roots but efficiently
expressed in leaves of tea plants. The expression level of CS gene in summer-grown leaves was much higher than in spring leaves.
Its expression level in leaves of the shaded or fertilized tea plants was significantly higher. Along the same shoot, CS gene
was expressed at much higher level in the young leaves (bud and the first leaf) than in the more mature second and third leaves.
RNA in situ hybridization indicated that tea CS gene was mainly expressed in the palisade parenchyma and the epicuticle of
leaves but less expressed in the spongy parenchyma and the hypoderm.
Published in Russian in Fiziologiya Rastenii, 2007, Vol. 54, No. 5, pp. 786–789.
The text was submitted by the authors in English. |
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Keywords: | Camellia sinensis tea caffeine synthase gene expression northern blot RNA in situ hybridization tea leaves |
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