Cell junctions in the subcommissural organ of the rabbit as revealed by use of ruthenium red |
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Authors: | Judith E. Kimble S. C. Sørensen K. Møllgaard |
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Affiliation: | (1) Laboratory of Electron microscopy, Anatomy Department A, University of Copenhagen, Copenhagen, Denmark;(2) Institute of Medical Physiology, Department A, University of Copenhagen, Copenhagen, Denmark |
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Abstract: | Summary Tight junctions were found in the apical junctional complex of the adult rabbit subcommissural organ (SCO) in addition to zonulae adhaerentes and gap junctions of typical ependymal cells. Ventricular perfusion of ruthenium red before fixation was found to give excellent results for distinguishing between gap and tight junctions at the ependymal surface. The implication of tight junctions as a mechanical means of sealing off the SCO area from the cerebrospinal fluid and the use of ruthenium red as a tracer substance are discussed.This work was supported by grants from Statens almindelige Videnskabsfond, Copenhagen. |
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Keywords: | Subcommissural organ (rabbit) Ependyma Cell junctions Ruthenium red |
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