Glycerol fomation inDunaliella cells under non-growing conditions with hypertonic lithium or magnesium media |
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Authors: | Shuhei Fujii Ryoichi Yamamoto Hideo Takada |
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Institution: | (1) Food Science Laboratory, Tezukayama Junior College, Gakuen-minami, 631 Nara |
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Abstract: | Glycerol formation ofDunaliella cells in non-growing media was investigated.Dunaliella tertiolecta andD. bioculata grew well in a NaCl medium but not at all in a LiCl or a MgCl2 medium. When the cells originally suspended in a medium containing 0.5 M NaCl were transferred to media which contained one
of 1 M NaCl, 1 M LiCl or 0.7 M MgCl2, the intracellular glycerol content increased.D. tertiolecta cultured in either a 1 M LiCl or a 0.7 M MgCl2 medium did not multiply, but maintained abilities to evolve O2 in the light and absorb O2 in thedark even after about a 5 day culture. From these results, it can be concluded that the halotolerance ofDunaliella to different kinds of salts is not directly related to osmoregulation by the glycerol formation. |
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Keywords: | Dunaliella bioculata D tertiolecta Glycerol formation Growth Halotolerance |
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