活性蛋白质表达谱分析与病毒学研究

活性蛋白质表达谱（activity-based protein profiling, ABPP）分析技术是功能蛋白质组学的一种策略，属于化学蛋白质组学的一部分.它借助化学小分子从功能角度直接切入蛋白质组的研究，能够直接对蛋白质组中感兴趣的靶酶蛋白的活性进行检测，为药物的发现提供强有力的支持.因此，ABPP技术被认为是基于功能的新一代蛋白质组学技术.随着ABPP分析技术和方法的不断成熟，其应用领域也不断扩展.最近一系列研究表明, 今后ABPP分析技术可能成为病毒学研究的又一重要武器.本文综述了ABPP分析技术的基本原理及其在病毒学研究中的应用.

Activity-based Protein Profiling in Virology Researches

Activity-based protein profiling（ABPP）is a powerful chemical proteomic strategy to characterize host enzyme functions for native complex biological systems in large scales. Using small probe molecules for investigating proteome, ABPP detects the activity of the interested protein directly, thus to provide evidence for drug discovery. With this new generation of functional proteomic technology, the host replication and proliferation of viruses can be continuously modulated and adjusted in vivo environments. A wide range of multifunctional viral proteins interplay with and modification of host cell proteins can be investigated, including most enzymes. With the upcoming improvements of the ABPP technology, the areas of its application would further extended as suggested by a series of recent studies. This review focused on the basic principles and applications of ABPP in virology research.