A Comparative Study of 4 Strains of Hartmannellid Amoebae |
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Authors: | ARTHUR H McINTOSH R SHIHMAN CHANG |
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Institution: | Boyce Thompson Institute, Yonkers, N. Y. 10701 and School of Medicine, Univ. of California, Davis, Calif. 95616 |
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Abstract: | SYNOPSIS. The biologic characteristics of 4 strains of hartmannellid amoebae were compared. All contained cytoplasmic DNA, degraded thymidine to CO2 and killed chick embryos at 32 C. All except H. glebae secreted a lipogenic toxin and contained antigen(s) capable of sensitizing erythrocytes. A. castellanii , unlike the other 3, also killed chick embryos at 37 C. The Fernald strain of H. rhysodes differed from the other 3 in its higher cytoplasmic grain count of cells labelled with 3H-thymidine and in its ability to induce the appearance of amoebic antigen within human cells. Increased nuclear labelling in the Fernald strain was observed with increasing concentrations of 3H-thymidine. |
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