| 假单胞菌酶法转化DL-ATC合成L-半胱氨酸 |
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| 引用本文： | 吴敏,陈蔚青,王普,何军邀.假单胞菌酶法转化DL-ATC合成L-半胱氨酸[J].氨基酸和生物资源,2009,31(1):58-63. |
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| 作者姓名： | 吴敏 陈蔚青 王普 何军邀 |
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| 作者单位： | 浙江工业大学生物制药研究所,浙江,杭州,310032;浙江树人大学生物与环境学院,浙江,杭州,310015 |
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| 基金项目： | 浙江省科技厅重大项目，浙江省重中之重学科开放基金 |
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| 摘 要： | 采用微生物酶转化法制备L-半胱氨酸具有周期短、成本低、区域和立体选择性强、反应条件容易控制、环境友好等特点,与传统的毛发水解以及化学合成工艺相比显示出明显的优越性。本文从假单胞菌产酶条件和酶学性质、DL-ATC生物转化途径、固定化细胞转化工艺、基因工程菌的研究、以及L-半胱氨酸脱巯基酶的研究等5个方面介绍了国内外关于生物转化DL-2-氨基-Δ2-噻唑啉-4-羧酸(DL-ATC)合成L-半胱氨酸的研究进展。
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| 关 键 词： | 假单胞菌 DL-ATC L-半胱氨酸 酶法转化 基因工程技术 L-半胱氨酸脱巯基酶 |
Progress on Enzymatic Synthesis of L- Cysteine from DL- ATC by Pseudomonas sp. |
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| WU Min,CHEN wei-qing,WANG Pu,HE Jun-yao.Progress on Enzymatic Synthesis of L- Cysteine from DL- ATC by Pseudomonas sp.[J].Amino Acids & Biotic Resources,2009,31(1):58-63. |
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| Authors: | WU Min CHEN wei-qing WANG Pu HE Jun-yao |
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| Institution: | WU Min, CHEN wei -qing ,WANG Pu ,HE Jun- yao (1 Institute of Biopharmaceutical Engineering, Zhejiang University of Technology,Hangzhou 310832; 2 School of Biological ＆ Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China) |
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| Abstract: | The microbial transformation method for L-cysteine production shows evident advantages,because of its short cycle time,low cost,high regio and stereoselectivity,easy control of reaction condition,and environment-friendly.Recently,studies on the bioconversion of DL-2-amino-△2-thiazoline-4-carboxylic acid(DL-ATC) to L-cysteine by intracellular enzymes were reported.The research progresses on L-cysteine production by microbial bioconversion,especially pseudomonas sp.,or its crude enzyme extract are summarized.The applications of immobilization technology in the biotransformation of DL-ATC to L-cysteine are introduced.The genetically engineered bacteria and the study progresses of L-cysteine desulfhydrase were also discussed. |
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| Keywords: | DL-ATC |
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