Preferable mitogenicity of beetle lectin, Allo a, for the blood cell culture of macaques and its influence on apoptosis |
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Authors: | Hiroshisa Hirai Kazuo Umetsu |
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Institution: | (1) Present address: Primate Research Institute, Kyoto University, Inuyama, 484-8506 Aichi;(2) Present address: Department of Forensic Medicine, Yamagata Univesity School of Medicine, Yamagata, 990-9585 Yamagata, Japan |
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Abstract: | Lectins with mitogenic properties on lymphocytes are requisite for chromosome research or cell biology using peripheral blood
cells. Though some lectins have been used as mitogens to stimulate lymphocytes in chromosome research of macaques, those reagents
have not always been optimum. At this time, an attempt was made to test the mitogenicity of lectin Allo A purified from the
beetle in Japanese and Rhesus monkeys. Medium with Allo A produced a significantly higher metaphase frequency and cell proliferation
(p=0.022) than that with Con A, which has previously yielded a higher quality. Dose effect experiments with ten monkeys revealed
that 10 μg/ml was the optimum dose to obtain higher proliferation in both lectins. Though intact blood samples usually have
many apoptotic cells, production of an optimum mitogen can be reduced in cell culture as an antagonistic result of higher
proliferation. In this connection as well, Allo A was superior to Con A. Thus, Allo A is probably the most useful lectin found
so far for obtaining a higher mitotic index of lymphocytes in Old World monkeys. |
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Keywords: | Macaques Lectin Allo A Mitogenicity Apoptosis |
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