| DNA克隆和组装技术研究进展 |
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| 引用本文： | 史晏榕,孙宇辉.DNA克隆和组装技术研究进展[J].微生物学通报,2015,42(11):2229-2237. |
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| 作者姓名： | 史晏榕 孙宇辉 |
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| 作者单位： | 武汉大学药学院 组合生物合成与新药发现教育部重点实验室 湖北 武汉 430071,武汉大学药学院 组合生物合成与新药发现教育部重点实验室 湖北 武汉 430071 |
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| 基金项目： | 国家973计划项目(No. 2012CB721005)；国家自然科学基金项目(No. 31470186) |
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| 摘 要： | DNA克隆和组装技术是重要的分子生物学工具。近年来，随着合成生物学的飞速发展，对大片段DNA元件的快速有效组装就显得尤为关键。同时，各种DNA克隆和组装技术也竞相发展起来。通过对基于非典型酶切连接、PCR、同源重组、单链退火拼接等原理发展起来的各种DNA克隆和组装技术进行综述，为合成生物学的进一步发展提供有效的操作工具。
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| 关 键 词： | DNA克隆和组装，非典型酶切连接，PCR，同源重组，单链退火拼接 |
Progress in DNA cloning and assembly techniques |
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| SHI Yan-Rong and SUN Yu-Hui.Progress in DNA cloning and assembly techniques[J].Microbiology,2015,42(11):2229-2237. |
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| Authors: | SHI Yan-Rong and SUN Yu-Hui |
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| Institution: | Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, School of Pharmaceutical Sciences, Wuhan University, Wuhan, Hubei 430071, China and Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, School of Pharmaceutical Sciences, Wuhan University, Wuhan, Hubei 430071, China |
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| Abstract: | DNA cloning and assembly techniques are essential tools for molecular biology research. With the recent advances in synthetic biology, efficient and fast assembly of large DNA including a number of genes is becoming more and more important. Meanwhile, a variety of DNA assembly methods are also developed very quickly. In this paper, various DNA assembly methods based on atypical enzyme digestion and ligation, PCR, homologous recombination, single strand annealing and splicing are summarized for providing effective technique tools for the further development of synthetic biology. |
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| Keywords: | DNA cloning and assembly Atypical enzyme digestion and ligation PCR Homologous recombination Single strand annealing and splicing |
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