The CRISPRdb database and tools to display CRISPRs and to generate dictionaries of spacers and repeats |
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Authors: | Ibtissem Grissa Gilles Vergnaud Christine Pourcel |
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Institution: | 1.Univ Paris-Sud, Institut de Génétique et Microbiologie, UMR 8621,Orsay,France;2.CNRS,Orsay,France;3.Centre d'Etudes du Bouchet,Vert le Petit,France |
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Abstract: | Background In Archeae and Bacteria, the repeated elements called CRISPRs for "clustered regularly interspaced short palindromic repeats"
are believed to participate in the defence against viruses. Short sequences called spacers are stored in-between repeated
elements. In the current model, motifs comprising spacers and repeats may target an invading DNA and lead to its degradation
through a proposed mechanism similar to RNA interference. Analysis of intra-species polymorphism shows that new motifs (one
spacer and one repeated element) are added in a polarised fashion. Although their principal characteristics have been described,
a lot remains to be discovered on the way CRISPRs are created and evolve. As new genome sequences become available it appears
necessary to develop automated scanning tools to make available CRISPRs related information and to facilitate additional investigations. |
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