Effects of osmotic swelling on voltage-gated calcium channel currents in rat anterior pituitary cells |
| |
Authors: | Ben-Tabou De-Leon Shlomo Blotnick Edna Nussinovitch Itzhak |
| |
Institution: | Department of Anatomy and Cell Biology, Hebrew University Hadassah Medical School, Jerusalem 91120, Israel. |
| |
Abstract: | Decrease in extracellular osmolarity (Os]e) results in stimulation of hormone secretion from pituitary cells. Different mechanisms can account for this stimulation of hormone secretion. In this study we examined the possibility that hyposmolarity directly modulates voltage-gated calcium influx in pituitary cells. The effects of hyposmolarity on L-type (IL) and T-type (IT) calcium currents in pituitary cells were investigated by using two hyposmotic stimuli, moderate (18-22% decrease in Os]e) and strong (31-32% decrease in Os]e). Exposure to moderate hyposmotic stimuli resulted in three response types in IL (a decrease, a biphasic effect, and an increase in IL) and in increase in IT. Exposure to strong hyposmotic stimuli resulted only in increases in both IL and IT. Similarly, in intact pituitary cells (perforated patch method), exposure to either moderate or strong hyposmotic stimuli resulted only in increases in both IL and IT. Thus it appears that the main effect of decrease in Os]e is increase in calcium channel currents. This increase was differential (IL were more sensitive than IT) and voltage independent. In addition, we show that these hyposmotic effects cannot be explained by activation of an anionic conductance or by an increase in cell membrane surface area. In conclusion, this study shows that hyposmotic swelling of pituitary cells can directly modulate voltage-gated calcium influx. This hyposmotic modulation of IL and IT may contribute to the previously reported hyposmotic stimulation of hormone secretion. The mechanisms underlying these hyposmotic effects and their possible physiological relevance are discussed. L-type channels; mechanosensitivity; somatotrophs; lactotrophs |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
| 点击此处可从《American journal of physiology》浏览原始摘要信息 |
| 点击此处可从《American journal of physiology》下载免费的PDF全文 |
|