Disruption of theRAD51 gene sensitizesS. cerevisiae cells to the toxic and mutagenic effects of hydrogen peroxide |
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Authors: | Z. Dudášová A. Dudáš A. Alemayehu D. Vlasáková E. Marková M. Chovanec V. Vlčková I. Brozmanová |
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Affiliation: | Laboratory of Molecular Genetics, Cancer Research Institute, Slovak Academy of Sciences, 833 91 Bratislava, Slovakia. |
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Abstract: | The RAD51 gene was disrupted in three different parental wild-type strains to yield three rad51 null strains with different genetic background. The rad51 mutation sensitizes yeast cells to the toxic and mutagenic effects of H2O2, suggesting that Rad51-mediated repair, similarly to that of RecA-mediated, is relevant to the repair of oxidative damage in S. cerevisiae. Moreover, pulsed-field gel electrophoresis analysis demonstrated that increased sensitivity of the rad51 mutant to H2O2 is accompanied by its decreased ability to repair double-strand breaks induced by this agent. Our results show that ScRad51 protects yeast cells from H2O2-induced DNA double-strand breakage. |
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