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Thulium Ion Promotes Apoptosis of Primary Mouse Bone Marrow Stromal Cells |
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| Authors: | Chunyan Dai Gong Chen Yaqiong Dong Jianlei Duan Shuxiang Wang Jinchao Zhang |
| Institution: | 1. College of Chemistry & Environmental Science, Chemical Biology Key Laboratory of Hebei Province, Hebei University, Baoding, People’s Republic of China 3. Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, College of Chemistry & Chemical Engineering, Hainan Normal University, Haikou, China 2. Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Hebei University, Baoding, 071002, China |
| Abstract: | Bone is one of the main target organs for the lanthanides (Ln). Biodistribution studies of Tm-based compounds in vivo showed that bone had significant uptake. But the effect of Tm3+ on primary mouse bone marrow stromal cells (BMSCs) has not been reported. So we investigated the effect and underlying mechanisms of Tm3+ on BMSCs. Cell viability, cell apoptosis, reactive oxygen species (ROS) level, lactate dehydrogenase (LDH) activity and mitochondrial membrane potential (MMP) were studied. The results indicated that Tm3+ increased the viability of BMSCs at concentrations of 1?×?10?7, 1?×?10?6, 1?×?10?5, and 1?×?10?4 mol/L in a dose-dependent manner, turned to decrease the viability of BMSCs at the highest concentration of 1?×?10?3 mol/L for 24, 48, and 72 h. Tm3+ at 1?×?10?3 mol/L promoted apoptosis of BMSCs, increased the ROS and LDH levels, and decreased MMP in BMSCs. Taken together, we demonstrated that Tm3+ at 1?×?10?3 mol/L might induce cellular apoptosis through mitochondrial pathway. These results may be helpful for more rational application of Tm-based compounds in the future. |
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