Study of the inhibition of alpha-amylase by the benzo[c]phenanthridine alkaloids sanguinarine and chelerythrine |
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Authors: | Zajoncova Ludmila Kosina Pavel Vicar Jaroslav Ulrichová Jitka Pec Pavel |
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Institution: | Department of Biochemistry, Faculty of Science, Palacky University, Slechtitel? 11, 783 71, Olomouc, Czech Republic. zajoncov@prfholnt.upol.cz |
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Abstract: | Inhibition of porcine pancreas and human saliva alpha-amylase (EC 3.2.1.1) by sanguinarine and chelerythrine was studied. The inhibition of alpha-amylase was assayed using a biosensor method which utilises a flow system equipped with a peroxide electrode. 250 microM sanguinarine and 250 microM chelerythrine cause complete inhibition of 1.9 nkat alpha-amylase from porcine pancreas. The same concentration of sanguinarine and chelerythrine caused 23.9% and 7.5% inhibition, respectively, of 1.9 nkat alpha-amylase from human saliva. Mixed type and partially reversible inhibition was found for both alpha-amylases treated with either alkaloid. |
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