| Immunogenicity and protective efficacy of recombinant M2e.Hsp70c（Hsp70359–610） fusion protein against influenza virus infection in mice |
| |
| 作者姓名： | Hamidreza Attaran ;Hassan Nili ;Majid Tebianian |
| |
| 作者单位： | [1]Avian Diseases Research Center, Faculty of Veterinary Medicine, University of Shiraz, Shiraz 71345- 1731, Iran; [2]Department of Clinical Studies, School of Veterinary Medicine, Shahrekord University, Shahrekord 88186/34141, Iran; [3]Department of Biotechnology and Immunology, Razi Vaccine and Serum Research Institute (RVSRI), Karaj 31975/148, Tehran, Iran |
| |
| 基金项目： | AKNOWLEDGMENTS The authors wish to thank Dr. Mehran Dabaghian and Dr. Mohammad Hossein Zabeh Jazi （faculty members of Razi Vaccine and Serum Research Institute）, Dr. Mokhtari （Virus Research Dept, Tehran University of Medical Science）, Dr. Seyyed Mahmood Ebrahimi （Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences） and Dr. Bin Gao （CAS Key Laboratory of Pathogenic Microbiology and Immunology, Chinese Academy of Sciences） for their helpful contribution to this project. |
| |
| 摘 要： | New strategies in vaccine development are urgently needed to combat emerging influenza viruses and to reduce the risk of pandemic disease surfacing. Being conserved, the M2 e protein, is a potential candidate for universal vaccine development against influenza A viruses. Mycobacterium tuberculosis Hsp70（mHsp70） is known to cultivate the function of immunogenic antigen-presenting cells, stimulate a strong cytotoxic T lymphocyte（CTL） response, and stop the induction of tolerance. Thus, in this study, a recombinant protein from the extracellular domain of influenza A virus matrix protein 2（M2e）, was fused to the C-terminus of Mycobacterium tuberculosis Hsp70（Hsp70c）, to generate a vaccine candidate. Humoral immune responses, IFN-γ-producing lymphocyte, and strong CTL activity were all induced to confirm the immunogenicity of M2 e.Hsp70c（Hsp70359–610）. And challenge tests showed protection against H1N1 and H9N2 strains in vaccinated groups. Finally these results demonstrates M2 e.Hsp70c fusion protein can be a candidate for a universal influenza A vaccine.
|
| 关 键 词： | 免疫原性 流感病毒 融合蛋白 病毒感染 C610 细胞毒性T淋巴细胞 重组 热休克蛋白70 |
| 收稿时间： | 2014 Apr 28 |
Immunogenicity and protective efficacy of recombinant M2e. Hsp70c (Hsp70359-610) fusion protein against influenza virus infection in mice |
| |
| Hamidreza Attaran,;Hassan Nili,;Majid Tebianian.Immunogenicity and protective efficacy of recombinant M2e. Hsp70c (Hsp70359-610) fusion protein against influenza virus infection in mice[J].Virologica Sinica,2014,29(4):218-227. |
| |
| Authors: | Hamidreza Attaran Hassan Nili Majid Tebianian |
| |
| Institution: | 1.Avian Diseases Research Center, Faculty of Veterinary Medicine, University of Shiraz, Shiraz 71345-1731, Iran2.Department of Clinical Studies, School of Veterinary Medicine, Shahrekord University, Shahrekord 88186/34141, Iran3.Department of Biotechnology and Immunology, Razi Vaccine and Serum Research Institute (RVSRI), Karaj 31975/148, Tehran, Iran |
| |
| Abstract: | New strategies in vaccine development are urgently needed to combat emerging influenza viruses and to reduce the risk of pandemic disease surfacing. Being conserved, the M2e protein, is a potential candidate for universal vaccine development against influenza A viruses. Mycobacterium tuberculosis Hsp70 (mHsp70) is known to cultivate the function of immunogenic antigenpresenting cells, stimulate a strong cytotoxic T lymphocyte (CTL) response, and stop the induction of tolerance. Thus, in this study, a recombinant protein from the extracellular domain of influenza A virus matrix protein 2 (M2e), was fused to the C-terminus of Mycobacterium tuberculosis Hsp70 (Hsp70c), to generate a vaccine candidate. Humoral immune responses, IFN-γ-producing lymphocyte, and strong CTL activity were all induced to confirm the immunogenicity of M2e.Hsp70c (Hsp70359–610). And challenge tests showed protection against H1N1 and H9N2 strains in vaccinated groups. Finally these results demonstrates M2e.Hsp70c fusion protein can be a candidate for a universal influenza A vaccine. |
| |
| Keywords: | influenza A virus M2e Hsp70 recombinant fusion protein universal influenza vaccine |
| 本文献已被 维普 SpringerLink 等数据库收录！ |
| 点击此处可从《中国病毒学》浏览原始摘要信息 |
| 点击此处可从《中国病毒学》下载免费的PDF全文 |
|
