Heterologous expression of human paraoxonases in <Emphasis Type="Italic">Pseudomonas aeruginosa</Emphasis> inhibits biofilm formation and decreases antibiotic resistance |
| |
Authors: | Fang Ma Yao Wang Yong Zhang Ning Xiong Baoyu Yang Shiyun Chen |
| |
Institution: | (1) Wuhan Institute of Virology, The Chinese Academy of Sciences, Wuhan, 430071, Hubei Province, China;(2) Graduate School of the Chinese Academy of Sciences, Beijing, 100049, China;(3) Present address: Shanghai Institute of Materia Medica, The Chinese Academy of Sciences, Shanghai, 201203, China |
| |
Abstract: | Quorum sensing (QS) regulates virulence and biofilm formation in Pseudomonas aeruginosa and other medically relevant bacteria. Human paraoxonases (hPONs) are a family of closely related enzymes with multiple functions,
including inactivation of the QS signal molecule in P. aeruginosa. However, there is no direct evidence to show the functions of hPONs on biofilm formation and antibiotic resistance in P. aeruginosa. In the present study, hPONs (hPON1, hPON2, and hPON3) genes were respectively cloned into the pMEKm12 shuttle vector and
transformed into P. aeruginosa strain PAO1. Expression of the three recombinant proteins was confirmed by Western blotting, and growth of the recombinant
strains was not affected by the hPONs gene expression. Biofilm formation and antibiotics resistance of the hPONs recombinant
strains were analyzed. Our results showed that biofilm formation was significantly inhibited in all of the three hPONs recombinant
strains. Interestingly, this inhibition can be reverted by addition of the corresponding hPONs polyclonal antibodies in the
culture media, further indicating that the inhibition of biofilm formation was due to hPONs protein expression. In addition,
we also demonstrated that hPONs expression decreased resistance of P. aeruginosa to gentamicin and ceftazidima, two antibiotics clinically used for the treatment of P. aeruginosa infection. |
| |
Keywords: | Pseudomonas aeruginosa Biofilm Antibiotic resistance Human paraoxonases Quorum sensing |
本文献已被 SpringerLink 等数据库收录! |
|