Extracellular calcium is involved in stomatal movement through the regulation of water channels in broad bean |
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Authors: | Hui-Min Yang Xiao-Yan Zhang Qing-Long Tang Gen-Xuan Wang |
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Institution: | (1) Key Laboratory of Grassland Agro-Ecosystems of Ministry of Agriculture, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730020, China;(2) School of Life Sciences, Lanzhou University, Lanzhou, 730000, China;(3) College of Life Science, Zhejiang University, Hangzhou, 310029, China |
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Abstract: | Involvement of extracellular Ca2+ in stomatal movement through the regulation of water channels was investigated in broad bean (Vicia faba L.). Leaf peels were first incubated to open stomata, and then transferred to buffers in the presence of different CaCl2 concentrations. Stomatal status was observed under magnification and stomatal aperture (pore width/length) was measured. Stomatal closure was significantly induced and aperture oscillation occurred at lower extracellular concentrations of calcium (Ca2+]ext), while at higher concentrations, no significant change in stomatal aperture was observed, which was similar to the response recorded with HgCl2. Lower Ca2+]ext-induced stomatal closure could be reversed using depolarizing buffer. It is suggested that lower Ca2+]ext regulates water channels through an indirect way and at higher concentrations, extracellular Ca2+ is involved in regulating stomatal aperture by directly influencing water channels to retard aperture change. |
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Keywords: | Ca2+ concentration Guard cell Vicia faba L Leaf peel Water channel |
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