Hexavalent molybdenum reduction to Mo-blue by <Emphasis Type="Italic">Acinetobacter calcoaceticus</Emphasis> |
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Authors: | M Y Shukor M F Rahman Z Suhaili N A Shamaan M A Syed |
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Institution: | (1) Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia |
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Abstract: | A local molybdenum-reducing bacterium was isolated and tentatively identified as Acinetobacter calcoaceticus strain Dr.Y12 based on carbon utilization profiles using Biolog GN plates and 16S rDNA comparative analysis. Molybdate reduction
was optimized under conditions of low dissolved oxygen (37 °C and pH 6.5). Of the electron donors tested, glucose, fructose,
maltose and sucrose supported molybdate reduction after 1 d of incubation, glucose and fructose supporting the highest Mo-blue
production. Optimum Mo-blue production was reached at 20 mmol/L molybdate and 5 mmol/L phosphate; increasing the phosphate
concentrations inhibited the production. An increase in an overall absorption profiles, especially at peak maximum at 865
nm and the shoulder at 700 nm, was observed in direct correlation with the increased in Mo-blue amounts. Metal ions, such
as chromium, cadmium, copper, mercury and lead (2 mmol/L final concentration) caused ≈88, 53, 80, 100, and 20 % inhibition,
respectively. Respiratory inhibitors, such as antimycin A, rotenone, sodium azide and cyanide showed in this bacterium no
inhibition of the Mo-blue production, suggesting that the electron transport system is not a site of molybdate reduction. |
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