Growth inhibition and excessive branching in <Emphasis Type="Italic">Aphanomyces cochlioides</Emphasis> induced by 2,4-diacetylphloroglucinol is linked to disruption of filamentous actin cytoskeleton in the hyphae |
| |
Authors: | Md Tofazzal Islam Yukiharu Fukushi |
| |
Institution: | (1) Laboratory of Ecological Chemistry, Graduate School of Agriculture, Hokkaido University, Kita-Ku, Sapporo 060-8589, Japan;(2) Present address: School of Agriculture and Rural Development, Bangladesh Open University, Gazipur, 1705, Bangladesh |
| |
Abstract: | We observed that 2,4-diacetylphloroglucinol (DAPG), a major antimicrobial metabolite produced by a rhizoplane bacterium Pseudomonas fluorescens ECO-001 inhibited mycelial growth of a damping-off phytopathogen Aphanomyces
cochlioides AC-5 through inducing excessive branching and curling in the hyphae. This study aimed to unravel the mode of action of DAPG
caused excessive branching, curling and growth inhibition of AC-5 hyphae by detecting localized changes in the cortical filamentous
actin (F-actin) organization by rhodamine-conjugated phalloidin. Confocal laser scanning microscopic observations revealed
that both living bacteria and DAPG severely disrupted the organization of F-actin in the A. cochlioides hyphae in a similar manner. Furthermore, an inhibitor of F-actin polymerization, latrunculin B also induced similar growth
inhibition, excessive branching and caused disruption of F-actin in the AC-5 hyphae. Our results suggested that growth inhibition
and excessive branching induced in A. cochlioides by DAPG is likely to be linked to the disruption of F-actin cytoskeleton in the affected hyphae. This is the first report
on disruption of cytoskeleton of a eukaryotic A. cochlioides by a well-known biocontrol metabolite DAPG secreted from a prokaryotic bacterium ECO-001. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|