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| 反相高效液相色谱法分离肝源性磷脂酰胆碱分子种 | |
| 引用本文： | 王亮,王波,辛毅,彭岩,张翠丽.反相高效液相色谱法分离肝源性磷脂酰胆碱分子种[J].天然产物研究与开发,2011,23(6):1144-1147. |
| 作者姓名： | 王亮 王波 辛毅 彭岩 张翠丽 |
| 作者单位： | 1. 大连医科大学生物技术系,大连,116044 2. 大连医科大学病理学教研室,大连,116044 3. 大连医科大学机能学实验室,大连,116044 |
| 摘 要： | 建立了反相高效液相色谱法(RP-HPLC)分离肝源性磷脂酰胆碱(PC)分子种的有效方法。考察了流动相中有机溶剂的种类、配比及流速对分离肝源性PC的影响。研究发现,在以甲醇为主的流动相中加入正己烷和醋酸铵有利于肝源性PC分子种的分离;甲醇-乙腈-水梯度洗脱不适于分离肝源性PC分子种。结果表明,采用Kromasil C18色谱柱(4.6 mm×200 mm,5μm),以甲醇-正己烷-0.05 mol/L醋酸铵-甘油(84∶6∶8∶0.6,体积比)为流动相,在流速1.0 mL/min、检测波长206 nm、柱温35℃的条件下,实现了肝源性PC各组分的分离。所建立的方法灵敏,重复性高,为进一步采用液质联用技术研究肝源性PC不同分子种的结构奠定基础。 |
| 关 键 词： | 高效液相色谱法 分离 肝源性磷脂酰胆碱 |
Separation of Phosphatidylcholine Molecules from Swine Liver by RP-HPLC |
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| WANG liang,WANG bo,XIN yi,PENG yan,ZHANG Cui-li.Separation of Phosphatidylcholine Molecules from Swine Liver by RP-HPLC[J].Natural Product Research and Development,2011,23(6):1144-1147. | |
| Authors: | WANG liang WANG bo XIN yi PENG yan ZHANG Cui-li |
| Institution: | WANG liang1,WANG bo2,XIN yi1,PENG yan3,ZHANG Cui-li1 1Bio-technology Department of Dalian Medical University,2Pathology Department of Dalian Medical University,3Functional Laboratory of Dalian Medical University,Dalian 116044,China |
| Abstract: | In this study an effective method for separating phosphatidylcholine(PC) molecules from swine liver was developed by using reversed-phase high performance liquid chromatography.The effect of organic modifiers,the composition of mobile phase and the flow rate on PC molecules separation were also investigated.The results showed that the separation process benefited by adding hexane and ammonium acetate into the mobile phase with methanol as the dominant solvent.The method of methanol-acetonitrile-water gradie... |
| Keywords: | high performance liquid chromatography separation and purification phosphatidylcholine from swine liver |
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