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| 吉林地区HFRS病原体基因型分析 | |
| 引用本文： | 赵臣,赵阳,李艳,袁忠海,孙可歆,张磊,罗恩杰. 吉林地区HFRS病原体基因型分析[J]. 微生物学杂志, 2011, 31(5): 101-104 |
| 作者姓名： | 赵臣 赵阳 李艳 袁忠海 孙可歆 张磊 罗恩杰 |
| 作者单位： | 1. 吉林医药学院检验系,吉林吉林,132013 2. 中国人民解放军第二二二医院传染科,吉林吉林,132012 3. 中国医科大学病原生物学教研室,辽宁沈阳,110001 |
| 摘 要： | 为调查吉林地区褐家鼠中汉坦病毒病原体基因型及其分布情况,采用免疫荧光法检测吉林市区及吉林市所属各县市收集的鼠肺标本,对汉坦病毒抗原阳性标本以巢式RT-PCR法扩增S基因片段上的核苷酸序列并测序,将扩增片段的核苷酸序列与已知病毒序列进行同源性分析及系统进化树分析.序列分析发现吉林地区褐家鼠携带的汉坦病毒均为汉城病毒,S3... |
| 关 键 词： | 肾综合征出血热(HFRS) 基因型 汉坦病毒 |
Genotype Analysis of HFRS Pathogen in Jilin Area |
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| ZHAO Chen,ZHAO Yang,LI Yan,YUAN Zhong-hai,SUN Ke-xin,ZHANG Lei,LUO En-jie. Genotype Analysis of HFRS Pathogen in Jilin Area[J]. Journal of Microbiology, 2011, 31(5): 101-104 | |
| Authors: | ZHAO Chen ZHAO Yang LI Yan YUAN Zhong-hai SUN Ke-xin ZHANG Lei LUO En-jie |
| Affiliation: | ZHAO Chen1,ZHAO Yang2,LI Yan1,YUAN Zhong-hai1,SUN Ke-xin1,ZHANG Lei1,LUO En-jie3(1.Dept.of Exam.,Jilin Med.Coll.,Jilin 132013,2.Dept.of Infect.PLA Hosp.222,Jilin 132012,3.Res.& Teach.Div.of Pathol.Biol.,Coll.of Basic Med.,China Med.Univ.,Shenyang 110001) |
| Abstract: | In order to investigate the pathogen genotypes and distribution of hantavirus carried by Norway rats(Rattus norvegicus) in Jilin area,the rat lung samples collected from Jilin downtown area,cities and towns within the jurisdiction of Jilin City were detected with indirect immunofluorescence assay.Nucleotide sequence of S gene segments of positive samples were amplified with nested RT-PCR and sequenced.The amplified nucleotide sequences were analyzed homologically and phylogenetically with known viral sequen... |
| Keywords: | HFRS genotype Hantavirus |
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