噬菌体抗体库技术的研究进展及应用

噬菌体抗体库技术是继噬菌体展示技术发展而来的一项基因抗体工程新技术。它可将含不同物种全部抗体可变区基因的基因库转化成展示在噬菌体表面的蛋白库,不仅使单克隆抗体的生产更方便、快速、高效地在体外进行,还开辟了单克隆抗体人源化的新途径,促进了人类单克隆抗体生产的发展。就近年来噬菌体抗体库技术的基因来源、发展关键及抗体应用的研究作一综述。

Progress on Phage Antibody Library Technology and Its Application

Phage antibody library is a new gene engineering antibody technology based on the phage display technology invented in 1990s.It converts all antibody variable genes of different species into populations of variant proteins displayed on the phage surface,which not only makes the generation of monoclonal antibody more convenient,rapid,efficient,but also sets up a new way of humanized monoclonal antibody,and promotes the development of human monoclonal antibody production.In this paper,the studies on source of genes,key of development,and antibody applications are reviewed.