| 甘草甜素对树突状细胞表型及生物学功能的影响 |
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| 引用本文： | 于洪涛,华慧,李伟伟,孟一鸣,李璇,张朕杰,单风平.甘草甜素对树突状细胞表型及生物学功能的影响[J].微生物学杂志,2011,31(5):35-39. |
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| 作者姓名： | 于洪涛 华慧 李伟伟 孟一鸣 李璇 张朕杰 单风平 |
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| 作者单位： | 中国医科大学免疫学教研室,辽宁沈阳,110001 |
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| 摘 要： | 培养小鼠髓系DC2．4细胞，加入LPS（阳性对照组）或甘草甜素，用扫描电镜观察DC的超微结构、流式细胞仪检测DC表面分子MHCII、CD86及CD40的表达、4-氨基安替比林（4-AAP）比色检测DC内酸性磷酸酶活性、ELISA方法检测DC培养上清中IL—12的浓度，体外刺激淋巴细胞增殖实验检测DC对同种异体T淋巴细胞的刺激能力。结果表明，与对照组相比，甘草甜素刺激后，DC表面树突状突起增多，表面分子MHCⅡ、CD86及CD40表达增加，酸性磷酸酶活性下降，培养上清中IL-12浓度升高，刺激同种异体T淋巴细胞的能力也明显增强。结果表明，甘草甜素能够促进小鼠髓系DC2.4表型及功能的成熟。
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| 关 键 词： | 甘草甜素 树突状细胞 表型 功能 成熟 |
Effects of Glycyrrhizin on Phenotypic and Biological Function of Dendritic Cells (DCs) |
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| YU Hong-tao,HUA Hui,LI Wei-wei,MENG Yi-ming,LI Xuan,ZHANG Zhen-jie,SHAN Feng-ping.Effects of Glycyrrhizin on Phenotypic and Biological Function of Dendritic Cells (DCs)[J].Journal of Microbiology,2011,31(5):35-39. |
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| Authors: | YU Hong-tao HUA Hui LI Wei-wei MENG Yi-ming LI Xuan ZHANG Zhen-jie SHAN Feng-ping |
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| Institution: | YU Hong-tao,HUA Hui,LI Wei-wei,MENG Yi-ming,LI Xuan,ZHANG Zhen-jie,SHAN Feng-ping(Teach.& Res.Div.of Immunol.,China Med.Uni.,Shenyang 110001) |
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| Abstract: | Myelogenous cells of mice DC2.4 were cultured and added with LPS(positive control group) or glycyrrhizin(GL),and observed with scanning electron microscope(SEM) for the DC ultra micro-structure;with flow cytometer to test the expression of surface molecules MHC II,CD86,and CD40;with 4-amino-antipyrin(4-AAP) chromometry to test the activity of acidic phosphatase inside the DC;with ELISA to test the concentration of IL-12 in the cultural supernatant of DC;with T-cell proliferation experiment stimulated in vit... |
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| Keywords: | glycyrrhizin dendritic cells phenotype function maturation |
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