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| 454测序技术在微生物生态学研究中的应用 | |
| 引用本文： | 段曌,肖炜,王永霞,赖泳红,崔晓龙. 454测序技术在微生物生态学研究中的应用[J]. 微生物学杂志, 2011, 31(5): 76-81 |
| 作者姓名： | 段曌 肖炜 王永霞 赖泳红 崔晓龙 |
| 作者单位： | 云南大学云南省微生物研究所,云南昆明,650091 |
| 基金项目： | 国家自然科学基金，云南省应用基础研究项目，云南省教育厅科学研究基金，云南大学理(工)科校级科研基金 |
| 摘 要： | 以Sanger法(双脱氧核苷酸末端终止法)为代表的第1代测序技术由于其成本高、速度慢、通量低等不足,满足不了大规模测序的要求.进入21世纪后,以Roche 454为代表的第2代测序技术诞生了,454测序法作为一种高通量的测序方法,近年来已被广泛应用于微生物生态学研究中.介绍了该测序技术的原理和操作步骤,结合本实验室的研... |
| 关 键 词： | 454测序 高通量测序 微生物多样性 宏基因组 |
Application of 454 Sequencing Technique in Microbial Ecology |
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| DUAN Zhao,XIAO Wei,WANG Yong-xi,LAI Yong-hong,CUI Xiao-long. Application of 454 Sequencing Technique in Microbial Ecology[J]. Journal of Microbiology, 2011, 31(5): 76-81 | |
| Authors: | DUAN Zhao XIAO Wei WANG Yong-xi LAI Yong-hong CUI Xiao-long |
| Affiliation: | DUAN Zhao,XIAO Wei,WANG Yong-xia,LAI Yong-hong,CUI Xiao-long(Yunnan Inst.of Microbiol.,Yunnan Uni.,Kunming 650091) |
| Abstract: | As the first generation sequencing technique Sanger method(dideoxy chain-termination method) could not meet the demand of the large-scale sequencing because of its high cost,slow speed,and low throughput.Roche 454 as one of the second generation DNA sequencing technology was emerged after entering 21st century,nowadays,as a high throughput sequencing it has been widely applied in microbial ecology.The principle and operating steps of the technique combining the research outcome in the lab were introduced in... |
| Keywords: | 454 sequencing high throughput sequencing microbial diversity macro-genome |
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