| 大豆根瘤菌HH103菌株培养基的筛选与优化 |
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| 引用本文： | 肖亦农,徐琼. 大豆根瘤菌HH103菌株培养基的筛选与优化[J]. 微生物学杂志, 2011, 31(6): 92-95 |
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| 作者姓名： | 肖亦农 徐琼 |
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| 作者单位： | 沈阳农业大学土地与环境学院,辽宁沈阳,110866 |
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| 基金项目： | 辽宁省自然科学基金，沈阳市科技局计划项目 |
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| 摘 要： | 以快生型大豆根瘤菌HH103菌株为供试菌株,采用单因素碳氮源利用试验和正交设计试验,确定最佳培养基及其配方。结果表明：该菌株在YMA中生长良好,最佳碳源为蔗糖,最佳氮源为酵母膏,最佳培养基成分配方（g/L）：蔗糖11,酵母膏0.9,K2HPO4 0.5,MnSO4 0.005,CaCl2 0.1,KH2PO4 0.5,MgSO4 0.2,KNO30.77,（NH4）2HPO4 0.33,FeCl3 0.005,pH 7.2。
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| 关 键 词： | 大豆根瘤菌 培养基筛选 培养基优化 正交试验 |
Medium Screening and Optimization for Soy Bean Rhizobium (Rhizobium fredii) HH103 |
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| XIA Yi-nong,XU Qiong. Medium Screening and Optimization for Soy Bean Rhizobium (Rhizobium fredii) HH103[J]. Journal of Microbiology, 2011, 31(6): 92-95 |
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| Authors: | XIA Yi-nong XU Qiong |
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| Affiliation: | XIA0 Yi-nong,XU Qiong |
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| Abstract: | Quick-growing soy bean rhizobium(Rhizobium fredii) HH103 was used as a tested strain,and adopted single factor nitrogen source utilization experiment and orthogonal design experiment to confirm the optimum medium and its formula.The results showed that the strain grew well on YMA,the optimum carbon source was sucrose,and the optimum nitrogen was yeast extract with the optimum medium formula as follows(in g/L): sucrose 11,yeast extract 0.9,K2HPO4 0.5,MnSO4 0.005,CaCl2 0.1,KH2PO4 0.5,MgSO4 0.2,KNO3 0.77,(NH4)2HPO4 0.33,FeCl3 0.005,and pH 7.2. |
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| Keywords: | soy bean rhizobium(Rhizobium fredii) medium screening and optimization orthogonal experiment |
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