| 蓝莓多糖BBP3-1的分离及结构分析 |
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| 引用本文： | 孙希云,刘宁,孟宪军,李斌,张琦.蓝莓多糖BBP3-1的分离及结构分析[J].天然产物研究与开发,2011,23(6):1080-1084. |
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| 作者姓名： | 孙希云 刘宁 孟宪军 李斌 张琦 |
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| 作者单位： | 1. 沈阳农业大学食品学院,沈阳,110161
2. 沈阳农业大学土地与环境学院,沈阳,110161 |
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| 基金项目： | 辽宁省主要浆果深加工项目(辽宁省科技厅项目)，特色小浆果精加工关键技术研究(辽宁省科技厅科技攻关项目) |
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| 摘 要： | 采用酶法优化进行水提醇沉得到蓝莓粗多糖.经双氧水脱色、酶解和三氯乙酸正丁醇结合法脱蛋白后通过DEAE Sepharose Fast Flow离子交换层析和sephacryl s- 300 HR凝胶过滤层析得到蓝莓多糖(BBP3-1).经紫外扫描显示无蛋白、核酸杂质.经高效液相色谱分析,BBP3-1的重均分子量Mw为18...
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| 关 键 词： | 蓝莓 多糖 分离 结构 |
Separation and Structural Analysis of Polysaccharides BBP3-1 from Blueberry |
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| SUN Xi-yun,LIU Ning,MENG Xian-jun,LI Bin,ZHANG Qi.Separation and Structural Analysis of Polysaccharides BBP3-1 from Blueberry[J].Natural Product Research and Development,2011,23(6):1080-1084. |
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| Authors: | SUN Xi-yun LIU Ning MENG Xian-jun LI Bin ZHANG Qi |
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| Institution: | SUN Xi-yun1,LIU Ning2,MENG Xian-jun1,LI Bin1,ZHANG Qi1 1Shenyang Agriculture University,College of Food,2Shenyang Agriculture University,College of Land and Environmental Science,Shenyang 110161,China |
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| Abstract: | In this study the raw polysaccharide was obtained from Blueberry by hot water extraction and ethanol precipitation method coupled with pectinase optimum.Then it was discolored by H2O2,enzymatic hydrolyzed and then removed of protein by combining trichloroacetic acid and n-butanol.Polysaccharide(BBP3-1) was purified using DEAE Sepharose Fast Flow ion exchange echromatography and Sephacryl S-300 HR gel filtration chromatography(GFC).UV spectra measurements confirmed there was no protein and nucleic acid.HPLC ... |
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| Keywords: | blueberry polysaccharide isolation structure |
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